User profiles for M. Holinstat

Michael Holinstat

University of Michigan
Verified email at
Cited by 2333

D Mehta, GU Ahmmed, BC Paria, M Holinstat… - Journal of Biological …, 2003 - ASBMB
We tested the hypothesis that RhoA, a monomeric GTP-binding protein, induces association
of inositol trisphosphate receptor (IP 3 R) with transient receptor potential channel (TRPC1),
and thereby activates store depletion-induced Ca 2+ entry in endothelial cells. We showed …


JN McLaughlin, L Shen, M Holinstat, JD Brooks… - Journal of biological …, 2005 - ASBMB
Gα12/13 activation, and changes in calcium reflect Gαq activation, implying that the
pharmacological differences between agonists are likely due to the ability of the receptor to
activate Gα12/13 or Gαq. This functional selectivity was quantitatively characterized by a …

M Holinstat, N Knezevic, M Broman, AM Samarel… - Journal of Biological …, 2006 - ASBMB
The interaction of endothelial cells with extracellular matrix proteins at focal adhesions sites
contributes to the integrity of vascular endothelial barrier. Although focal adhesion kinase
(FAK) activation is required for the recovery of the barrier function after increased endothelial …

M Holinstat, D Mehta, T Kozasa, RD Minshall… - Journal of Biological …, 2003 - ASBMB
Heterotrimeric G-proteins of the Gα 12/13 family activate Rho GTPase through the guanine
nucleotide exchange factor p115RhoGEF. Because Rho activation is also dependent on
protein kinase Cα (PKCα), we addressed the possibility that PKCα can also induce Rho …

GU Ahmmed, D Mehta, S Vogel, M Holinstat… - Journal of Biological …, 2004 - ASBMB
The TRPC1 (t ransient r eceptor p otential c anonical-1) channel is a constituent of the
nonselective cation channel that mediates Ca 2+ entry through store-operated channels
(SOCs) in human endothelial cells. We investigated the role of protein kinase Cα (PKCα) …

M Holinstat, B Voss, ML Bilodeau… - Journal of Biological …, 2006 - ASBMB
Regulation of platelet activation plays a central role in hemostasis and pathophysiological
processes such as coronary artery disease. Thrombin is the most potent activator of
platelets. Human platelets express two thrombin receptors, PAR1 and PAR4, both of which …

LC Edelstein, LM Simon, RT Montoya, M Holinstat… - Nature medicine, 2013 -
Racial differences in the pathophysiology of atherothrombosis are poorly understood. We
explored the function and transcriptome of platelets in healthy black (n= 70) and white (n=
84) subjects. Platelet aggregation and calcium mobilization induced by the PAR4 thrombin …

…, X Kong, L Ma, P Fortina, S Kunapuli, M Holinstat… - Blood, 2014 - Am Soc Hematology
There is little data considering relationships among human RNA, demographic variables
and primary human cell physiology. The Platelet RNA And eXpression-1 (PRAX1) study
measured platelet aggregation to arachidonic acid, ADP, PAR1-AP and PAR4-AP, as well …

…, R Sandoval, RD Minshall, M Holinstat… - The Journal of …, 2002 - Wiley Online Library
The adhesive force generated by the interaction of integrin receptors with extracellular
matrix (ECM) at the focal adhesion complex may regulate endothelial cell shape, and
thereby the endothelial barrier function. We studied the role of focal adhesion kinase (FAK) …


B Voss, JN McLaughlin, M Holinstat, R Zent… - Molecular …, 2007 - ASPET
Thrombin-mediated activation of platelets is critical for hemostasis, but the signaling
pathways responsible for this process are not completely understood. Additionally, signaling
within this cascade can also lead to thrombosis. In this study we have defined a new …